Sensitivity: The minimum detectable dose of Rat PCSK9 was determined to be approximately 47 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 47 pg/ml
Sensitivity: The minimum detectable dose of Rhesus CD32a / Fc gamma RIIA / FCGR2A was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 7.8 pg/ml
Sensitivity: The minimum detectable dose of HIV-1 gp120 / Glycoprotein 120 was determined to be approximately 39.06 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 39.06 pg/ml
Sensitivity: The minimum detectable dose of Human HER2 / ErbB2 / CD340 was determined to be approximately 24 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 24 pg/ml
Sensitivity: The minimum detectable dose of Rhesus CD16 / FCGR3 was determined to be approximately 12.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 12.5 pg/ml
Sensitivity: The minimum detectable dose of Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 62.5 pg/ml
Sensitivity: The minimum detectable dose of Canine KIM-1 / TIM1 / HAVCR1 was determined to be approximately 125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 125 pg/ml
Sensitivity: The minimum detectable dose of Ferret TNF-alpha / TNFA was determined to be approximately 156.25 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 156.25 pg/ml
Sensitivity: The minimum detectable dose of Ferret CD4 / Leu-3 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 31.25 pg/ml
Sensitivity: The minimum detectable dose of Human CD69 was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 15.6 pg/ml
Sensitivity: The minimum detectable dose of Human SIGLEC5 was determined to be approximately 234.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 234.38 pg/ml
Sensitivity: The minimum detectable dose of Human IL3RA / CD123 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 31.25 pg/ml
Seit 1971 dient der ELISA dem Nachweis und oder der Konzentrationsbestimmung von Proteinen, eines Analytgemisches, niedermolkularer Verbindungen (zb. Toxine, Hormone) oder Viren.
Der ELISA beruht auf einem Antikörper-Antigen-Nachweis. Grundlage hierbei ist, dass entweder Antigen oder Antikörper auf einer Multiwell-Platte (z. aus Polystyrol) immobilisiert werden und das nachzuweisende Analyt im Folgeschritt gebunden wird.
Die Antigene werden über Wasserstoffbrückenbindungen, ionische und hydrophobe Wechselwirkungen und Van-der-Waals-Kräfte von den Antikörpern gebunden. Dabei erfolgt diese Bindung nach dem Schlüssel-Schloss-Prinzip und ist sehr spezifisch. Die verwendeten Antikörper können dabei Monoklonal (mAb) oder Polyklonal sein. Monoklonale Antikörper werden aus Hybridoma-Zellen, einer Fusion aus Krebszelle (Myelomazelle) und antikörperproduzierender Zelle (B-Zelle) hergestellt und binden selektiv ein spezifisches Epitop auf dem Antigen. Polyklonale Antikörper sind ein Gemisch verschiedener Antikörper, die verschiedene Epitope auf dem Antigen erkennen. Nach erfolgreicher Bindung erfolgt der Nachweis in der Regel kolorimetrisch.
Dabei kommt es nach erfolgreicher Antigen-Antikörper-Bindung und Zugabe eines Substrates zu einem Farbumschlag in der Mikrotiterplatte, welches durch ein Enzym katalysiert wird, das zuvor mit dem Antikörper oder Antigen konjugiert wurde.
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