Escherichia coli (E. coli) acnB ELISA Kit

Brand

Sino Biological

short description

For the quantitative determination of E. coli acnB concentration in cell lysate.

spezie

E. coli

natural samples' content determination

The concentration of AcnB detected in E.coli cell lysate is 85mg/mL.The E.coli cell lysate' total protein concentration was about 10mg/mL.

Cross Reaction

This assay recognizes both recombinant and natural AcnB. A Baculovirus lysate which total protein concentration was about 0.1mg/mL was detected by this assay. There was no AcnB was detected.A 293 cell lysate which total protein concentration was about 6mg/mL was detected by this assay. There was no AcnB was detected.

detection target

acnB
Antigene: 13102-ENAE
capture antibody type: mouse mAbdetection antibody type

sensitivity

32.4 pg/ml

detection range

78.1-5000 pg/ml

certificate

ISO9001-2008

pack size

96 Wells

storage and Stability

Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA

Principle of the Elisa

Solid Phase Sandwich ELISA

Kit Content

1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution