For the quantitative determination of E. coli acnB concentration in cell lysate.
The concentration of AcnB detected in E.coli cell lysate is 85mg/mL.The E.coli cell lysate' total protein concentration was about 10mg/mL.
This assay recognizes both recombinant and natural AcnB. A Baculovirus lysate which total protein concentration was about 0.1mg/mL was detected by this assay. There was no AcnB was detected.A 293 cell lysate which total protein concentration was about 6mg/mL was detected by this assay. There was no AcnB was detected.
acnBAntigene: 13102-ENAEcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Specific Against||E. coli|
|Antigen Name||Escherichia coli (E. coli) acnB|
|Detection Range||78.1-5000 pg/ml|
|Alias||Escherichia coli (E. coli) acnB|