ULTRASENSITIVE THYROID STIMULATING HORMONE (U-TSH) ENZYME IMMUNOASSAY TEST KIT

Brand

GENWAY

Overview

Intended Use: For the quantitative determination of thyroid stimulating hormone (TSH) concentration in human serum. Introduction: The determination of serum or plasma levels of thyroid stimulating hormone (TSH or thyrotropin) is recognized as a sensitive method in the diagnosis of primary and secondary hypothyroidism. ^1 TSH is secreted by the anterior lobe of the pituitary gland and induces the production and release of thyroxine (T4) and triiodothyronine (T3) from the thyroid gland. ^2 It is a glycoprotein with a molecular weight of approximately 28 000 daltons consisting of two chemically different subunits alpha and beta. ^3Although the concentration of TSH in the blood is extremely low it is essential for the maintenance of normal thyroid function. The release of TSH is regulated by a TSH-releasing hormone (TRH) produced by the hypothalamus. The levels of TSH and TRH are inversely related to the level of thyroid hormone. When there is a high level of thyroid hormone in the blood less TRH is released by the hypothalamus so less TSH is secreted by the pituitary. The opposite action will occur when there is decreased thyroid hormone in the blood. This process is known as a negative feedback mechanism and is responsible for maintaining the proper blood levels of these hormones. ^4 5TSH and the pituitary glycoproteins: luteinizing hormone (LH) follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG) have identical alpha chains. ^2 The beta chains are distinct but do contain regions with identical amino acid sequences. These regions of homology can cause considerable cross-reactivity with some polyclonal TSH antisera. The use of a monoclonal antibody in Biotech\'s TSH ELISA test eliminates this interference which could result in falsely elevated TSH values in either menopausal or pregnant females a population whose evaluation of thyroid status is clinically significant. ^6 7 8Principles of the assay: The Biotech TSH ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. ^9 10 The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact TSH molecule. Mouse monoclonal anti-TSH antibody is used for solid phase immobilization (microtiter wells) and goat anti-TSH antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the two antibodies resulting in the TSH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 60-minute or overnight incubation at room temperature the solid phase is washed with water to remove unbound labeled antibodies. A solution of 3 3\' 5 5\'-Tetramethylbenzidine (TMB) is added and incubated for 20 minutes resulting in the development of a blue color. The color development is stopped with the addition of 1N HCl and the resulting yellow color is measured spectrophotometrically at 450 nm. The concentration of TSH is directly proportional to the color intensity of the test sample. Storage and Stability: 1. Store the unopened kit at 2-8C upon receipt and when it is not in use until the expiration shown on the kit label. Refer to the package label for the expiration date. 2. The opened and used reagents are stable until the expiration date if stored properly at 2-8C. 3. Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air. Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice. 2. Serum samples demonstrating gross lipemia gross hemolysis or turbidity should not be used with this test. 3. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. 4. The Biotech TSH assay has not been tested on newborns and is not for use in screening newborns. Product Name: Thyroid Stimulating Hormone (TSH) Subunit: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin lutropin follitropin and gonadotropin. Subcellular Location: Secreted. Similarity: Belongs to the glycoprotein hormones subunit alpha family.

spezie

Canine

detection target

ULTRASENSITIVE THYROID STIMULATING HORMONE (U-TSH)

pack size

1x96 Wells