Schistosoma mansoni IgG

Brand

GENWAY

Overview

Intended Use: The Schistosoma mansoni IgG-ELISA is intended for the qualitative determination of IgG class antibodies against Schistosoma mansoni in human serum or plasma (citrate). Introduction: Schistosomes belong to the class of distomas (trematodes). They rank among the most frequent pathogens. Estimations originate in more than 200 million affected people. The mature parasites are 6 ? 22 mm long. The most important species are Schistosoma mansoni S. japonicum and S. haematobium. Schistosoma mansoni is common in Africa South America and Middle East. Schistosomiasis (bilharziosis) is ? depending on species and location of the parasites ? a disease of the intestine liver and spleen resp. urinary passages. Humans are (re)infected by contact with fresh water which is contaminated by ova containing urine or faeces. If larvae bore into human skin first a transient skin reaction appears (itch with exanthema or erythema by repeatedly infection cercarial dermatitis is possible). After 3 - 10 weeks the meanwhile sexually mature worms synthesize cytotoxic and allergic substances which course feverish reaction in humans (Katayama fever). The infected person is mostly harmed by the eggs which get into organs via blood excreting proteins and glycoproteins. The person reacts under participation of own antibodies and immune complexes with formation of granuloma and granulomatous proliferation in intestine and urinary bladder mucosa. Not excreted eggs die after 3 weeks and will be dissolved or calcified. The affected tissue gets fibrous. In final stage bilharziosis will cause dead. Principles of the assay: The qualitative immunoenzymatic determination of IgG-class antibodies against Schistosoma mansoni is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Schistosoma mansoni antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled Protein A conjugate is added. This conjugate binds to antigen-antibody complexes. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Schistosoma-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader. Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2. . . 8 °C. Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history symptomatology as well as serological data. In immunocompromised patients and newborns serological data only have restricted value. References Burkhardt F. [Hrsg. ]: Mikrobiologische Diagnostik. Thieme Stuttgart New York 1992. 9-452 Hahn; Falke; Kaufmann; Ullmann [Hrsg. ]: Medizinische Mikrobiologie und Infektiologie. Springer Berlin Heidelberg 32001. 784ff. Jordan P. ; Webbe G. ; Sturrock F. : Human schistosomiasis. CAB International Wallingford Oxon 1993. Lang W. ; Löscher T. [Hrsg. ]: In: Tropenmedizin in Klinik und Praxis. Thieme Stuttgart New York 2000. 9-204http://www. rki. de/INFEKT/STECKBRF/STBR_PA/PARASIT. HTM?/INFEKT/STECKBRF/STBR_PA/SCHISTO. HTM&1

spezie

other

detection target

Schistosoma mansoni IgG

pack size

1x96 assay