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Boster
For quantitative detection of rat IL-6 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Rat
There is no detectable cross-reactivity with other relevant proteins.
Rat IL-6Protein name: Interleukin-6Gene Full Name: interleukin 6abbreviation: IL6Background: Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6(IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone(CRH). Interleukin-6(IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor(IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa. ID:P20607 ID:24498
B cell differentiation factor | B cell stimulatory factor 2| B-cell stimulatory factor 2| BSF 2| BSF-2| BSF2| CDF| CTL differentiation factor| Cytotoxic T cell differentiation factor | Hepatocyte stimulating fact| HGF| HPGF| HSF | Hybridoma growth factor| Hybridoma plasmacytoma growth factor | IFN-beta-2| IFNB2 | IL 6| IL-6| IL6| IL6 protein | IL6_HUMAN| Interferon beta 2| Interferon beta-2| Interleukin 6| Interleukin 6 (interferon beta 2) | Interleukin BSF 2| Interleukin-6| Interleukin6
Nativ and recombinant rat IL-6
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, F25-T211
<5pg/ml
62.5pg/ml-4000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|150|392|1635|176|415|1474 Standard deviation|13.65|34.89|91.56|16.72|38.18|110.55 CV(%)|9.1|8.9|5.6|9.5|9.2|7.5
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s rat IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, F25-T211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-6 amount of sample captured in plate.
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml rat IL-6 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each rat IL-6 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.036|0.082|0.140|0.198|0.373|0.751|1.292|2.032
Catalog number|Description|Quantity-|96-well plate precoated with anti- rat IL-6 antibody|1ST0000-10|Lyophilized recombinant rat IL-6 standard|10ng/tubex2AR1107|Biotinylated anti- rat IL-6 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
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Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <5pg/ml |
Specific Against | Rat |
Antigen Name | Interleukin-6 (IL6) |
Detection Range | 62.5pg/ml-4000pg/ml |
Alias | B cell differentiation factor , B cell stimulatory factor 2, B-cell stimulatory factor 2, BSF 2, BSF-2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor , Hepatocyte stimulating fact, HGF, HPGF, HSF , Hybridoma |