Rat IL-4 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat IL-4 in cell culture supernates and serum.

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Rat IL-4
Protein name: Interleukin-4
Gene Full Name: interleukin 4
abbreviation: IL4
Background: Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.
ID:P20096
ID:287287

alias

B cell growth factor 1| B cell stimulatory factor 1| BCGF 1| BCGF1| Binetrakin| BSF 1| BSF1| IL 4| Interleukin 4| Lymphocyte stimulatory factor 1| MGC79402| Pitrakinra

Origin

Nativ and recombinant rat IL-4

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, C25-S147

sensitivity

<1pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|54.7|428|738|53.8|387|729
Standard deviation|3.23|21.83|31.73|4.79|25.93|45.2
CV(%)|5.9|5.1|4.3|8.9|6.7|6.2

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat IL-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-4 has been precoated onto 96-well plates. Standards(E.coli, C25-S147) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-4 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat IL-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each rat IL-4 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.057|0.106|0.159|0.263|0.517|1.043|1.781|2.383

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat IL-4 antibody|1
ST0000-10|Lyophilized recombinant rat IL-4 standard|10ng/tubex2
AR1107|Biotinylated anti- rat IL-4 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4