BioAim Scientific (Kanada)
The Bioaim Rat IL-4 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-4 in rat serum, plasma and cell culture media. An antibody specific for rat IL-4 was coated on a 96-well plate. Standards and samples are added to the wells and any IL-4 present binds to the immobilized antibody. The wells are washed and biotinylated anti-rat IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-4 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
interleukin 4(IL-4) Background: The interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to Interleukin 13.
< 1 pg/ml
Serum, plasma, Cell culture supernatant
|Sensitivity||< 1 pg/ml|
|Antigen Name||interleukin 4|
|Detection Range||0.82-200 pg/ml|