Rat CD93/C1qR ELISA Pair Set

Brand

Sino Biological

short description

The Rat CD93/C1qR ELISA Pair Set is for the quantitative determination of Rat CD93/C1qR. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Rat CD93/C1qR coated on a 96-well plate. Standards and samples are added to the wells, and any Rat CD93/C1qR present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Rat CD93/C1qR polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Rat CD93/C1qR present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Rat

detection target

CD93
Standard (antigene): Each vial contains 40 ng of recombinant Rat CD93/C1qR. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
capture antibody type: 1 mg/mL of rabbit anti-Rat CD93/C1qR monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in PBS before coating. (Catalog: # 80207-R158)
detection antibody type: 0.2 mg/mL of rabbit anti-Rat CD93/C1qR polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4°C;). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use.
Lable: HRP

sensitivity

The minimum detectable dose of Rat CD93/C1qR was determined to be approximately 15.625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

15.625 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.