Rat Cadherin-2/N-Cadherin PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat Cadherin-2 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

rat Cadherin-2
Protein name: Cadherin-2
Gene Full Name: cadherin 2, type 1, N-cadherin (neuronal)
abbreviation: CDH2
Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
ID:Q9Z1Y3
ID:83501

alias

CDw325|CDHN|Neural cadherin|N-cadherin|CD325

Origin

Nativ and recombinant rat Cadherin-2

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, D160-A724

sensitivity

<20pg/ml

detection range

0.78ng/ml-50ng/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|9.8|23.7|39.5|13.5|28.7|41.6
Standard deviation|0.451|1.256|1.738|0.864|2.066|2.329
CV(%)|4.6|5.3|4.4|6.4|7.2|5.6

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat Cadherin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cadherin-2 has been precoated onto 96-well plates. Standards(NSO, D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Cadherin-2 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml rat Cadherin-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each rat Cadherin-2 standard solution and each sample is measured in duplicate.

tipical result

Concentration(ng/ml)|0|0.78|1.56|3.12|6.25|12.5|25|50 O.D.|0.179|0.230|0.259|0.323|0.474|0.817|1.264|1.825

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat Cadherin-2 antibody|1
ST0000-50|Lyophilized recombinant rat Cadherin-2 standard|50ng/tube�2
AR1107|Biotinylated anti- rat Cadherin-2 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4