Rat beta IG-H3/TGFBI PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat beta IG-H3 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

alias

Beta ig | Beta ig h3 | Beta ig-h3 | Big h3 | BIGH3 | CDB1 | CDG2 | CDGG1 | CSD | CSD1 | CSD2 | CSD3 | EBMD | Kerato epithelin | Kerato-epithelin | LCD1 | RGD CAP | RGD-CAP | TGFBI | Q15582

Origin

Natural and recombinant rat beta IG-H3

Immunogene

NSO, G24-G685

sensitivity

<15pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|451|1349|5410|563|1588|5523 Standard deviation|18.49|70.15|373.29|24.21|87.34|397.66 CV(%)|4.1|5.2|6.9|4.3|5.5|7.2

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Bosterﾒs rat beta IG-H3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for beta IG-H3 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: G24-G685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for beta IG-H3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat beta IG-H3 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat beta IG-H3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, cell lysates, serum or plasma (heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each rat beta IG-H3 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.010|0.031|0.064|0.137|0.376|0.922|1.879|2.599

Kit Content

Catalog number|Description|Quantity EK1571-CAP|96-well plate precoated with anti- rat beta IG-H3antibody|1 EK1571-ST|Lyophilized recombinant rat beta IG-H3 standard|10ng/tube�2 EK1571-DA|Biotinylated anti- rat beta IG-H3 antibody|130?l(dilution 1:100) AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130?l(dilution 1:100) AR1106-1|Sample diluent buffer|30ml AR1106-2|Antibody diluent buffer|12ml AR1106-3|ABC diluent buffer|12ml AR1104|TMB color developing agent|10ml AR1105|TMB stop solution|10ml PLA-SEA|Adhesive cover|4