Rat Angiopoietin-2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat Angiopoietin-2 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

alias

ANG 2 | ANG2 | ANG-2 | Angiopoietin 2 | Angiopoietin2 | Angiopoietin-2 | Angiopoietin2(ANG2) | Angiopoietin-2(ANG-2) | ANGP2 | ANGPT2 | O15123

Origin

Natural and recombinant rat Angiopoietin-2

Immunogene

NSO, Y19-F496

sensitivity

<15pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|417|1330|5235|504|1461|5560 Standard deviation|16.26|59.85|266.99|28.73|94.97|422.56 CV(%)|3.9|4.5|5.1|5.7|6.5|7.6

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Bosterﾒs rat Angiopoietin-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Y19-F496) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Angiopoietin-2 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, cell lysates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each rat Angiopoietin-2 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.032|0.085|0.143|0.248|0.423|0.816|1.359|2.231

Kit Content

Catalog number|Description|Quantity EK1574-CAP|96-well plate precoated with anti- rat Angiopoietin-2 antibody|1 EK1574-ST|Lyophilized recombinant rat Angiopoietin-2 standard|10ng/tube�2 EK1574-DA|Biotinylated anti- rat Angiopoietin-2 antibody|130?l(dilution 1:100) AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130?l(dilution 1:100) AR1106-1|Sample diluent buffer|30ml AR1106-2|Antibody diluent buffer|12ml AR1106-3|ABC diluent buffer|12ml AR1104|TMB color developing agent|10ml AR1105|TMB stop solution|10ml PLA-SEA|Adhesive cover|4