Rat Angiopoietin-1 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat Angiopoietin-1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

rat Angiopoietin-1
Protein name: Angiopoietin-1
Gene Full Name: angiopoietin 1
abbreviation: ANGPT1
Background: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
ID:O35460
ID:89807

alias

1110046O21Rik| AGP 1| AGP1| AGPT| ANG 1| ANG1| Angiopoietin1| ANGPT 1| ANGPT1| KIAA0003

Origin

Nativ and recombinant rat Angiopoietin-1

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, H16-F498

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.31|3.64|6.95|1.42|3.75|7.03
Standard deviation|0.046|0.138|0.292|0.058|0.173|0.401
CV(%)|3.5|3.8|4.2|4.1|4.6|5.7

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat Angiopoietin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-1 has been precoated onto 96-well plates. Standards(NSO, H16-F498) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Angiopoietin-1 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml rat Angiopoietin-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each rat Angiopoietin-1 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|313|625|1250|2500|5000|10000 O.D.|0.151|0.192|0.247|0.329|0.522|0.868|1.421|2.159

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat Angiopoietin-1 antibody|1
ST0000-10|Lyophilized recombinant rat Angiopoietin-1 standard|10ng/tube�2
AR1107|Biotinylated anti- rat Angiopoietin-1 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4