Rat ADAM12 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat ADAM12 in cell culture supernates, serum and plasma(heparin).

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

rat ADAM12
Protein name: Disintegrin and metalloproteinase domain-containing protein 12
Gene Full Name: ADAM metallopeptidase domain 12
abbreviation: ADAM12
Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.
ID:F1M9Z9
ID:679837

alias

ADA12_HUMAN| ADAM 12| ADAM metallopeptidase domain 12| ADAM12| Disintegrin and metalloproteinase domain-containing protein 12| MCMP| MCMPMltna| Meltrin-alpha| MLTN| MLTNA

Origin

Nativ and recombinant rat ADAM12

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

CHO, R29-S513

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.46|3.65|6.89|1.55|3.81|6.92
Standard deviation|0.066|0.139|0.234|0.088|0.175|0.324
CV(%)|4.5|3.8|3.4|5.7|4.6|4.7

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat ADAM12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM12 has been precoated onto 96-well plates. Standards(CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat ADAM12 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat ADAM12 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each rat ADAM12 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.033|0.055|0.111|0.207|0.399|0.735|1.251|2.029

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat ADAM12 antibody|1
ST0000-10|Lyophilized recombinant rat ADAM12 standard|10ng/tube�2
AR1107|Biotinylated anti- rat ADAM12 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4