OxiSelect™ BPDE Protein Adduct ELISA Kit

Brand

Cell Biolabs

short description

For detection and quantitation of benzo(a)pyrene diol epoxide (BPDE) protein adducts
BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC.  The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody.  The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.
Research area: Oxidative Stress/ Damage
Research focus Protein Oxidation / Nitration

spezie

other

detection target

BPDE Protein Adduct

sample type

Suitable for use with protein samples such as purified protein, plasma, serum, or cell lysates

pack size

96 assays

storage and Stability

Upon receipt, aliquot and store the Reduced BSA and BPDE-BSA Standards at -20°C to avoid multiple freeze/thaw cycles. Store all other kit components at 4°C.

Shipping Temp

Blue Ice

Kit Content

BPDE-BSA standard included