Mouse TSLP ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse TSLP ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TSLP in mouse serum, plasma and cell culture media. An antibody specific for mouse TSLP was coated on a 96-well plate. Standards and samples are added to the wells and any TSLP present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse TSLP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TSLP present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cell growth; proliferation;

specific against

Mouse

detection target

thymic stromal lymphopoietin(TSLP)
Background: Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM– pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of myeloid (CD11c+) dendritic cells. TSLP has also been shown to activate the maturation of a specific subset of dendritic cells located within the epidermis, called Langerhans cells. Within the thymus TSLP activation of both myeloid and plasmacytoid (CD123+) dendritic cells results in the production of regulatory T cellsTSLP R, also named Delta and CRLM-2 , was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified as a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R.

sensitivity

< 1 pg/ml

detection range

0.82-200 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q9JIE6