Mouse TNF-alpha ELISA Kit

Brand

Cell Sciences

Description

The Cell Sciences Mouse TNF-alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-alpha in serum, plasma and cell culture supernatants.This assay employs an antibody specific for Mouse TNF-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TNF-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

spezie

Mouse

Cross Reaction

Mouse

detection target

Mouse TNF-alpha
Antigen: TNF-alpha

alias

DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, tumor necrosis factor, TNF-a, cachectin, APC1 protein, TNF, monocyte-derived, TNF, macrophage-derived, TNF superfamily, member 2, tumor necrosis factor alpha, tumor necrosis factor ligand superfamily member 2

sample size

1

pack size

PP

UNSPSC

41116126 and E