Mouse Prolactin ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse Prolactin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Prolactin in mouse serum, plasma and cell culture media. An antibody specific for mouse Prolactin was coated on a 96-well plate. Standards and samples are added to the wells and any Prolactin present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Prolactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Prolactin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker; Aging; Peptide Hormone;

specific against

Mouse

detection target

Prolactin ( Prolactin )
Background: Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family. Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. It is associated with human milk production. In fish it is thought to be related to control of water and salt balance. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. It has important cell cycle-related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor, binding to cytokine-like receptors, it influences hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways. The hormone acts in endocrine, autocrine and paracrine manner through the prolactin receptor and a large number of cytokine receptors.

alias

Prl1a1, AV290867, Gha1

sensitivity

20 pg/ml

detection range

27.4-20000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P06879