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Boster
For quantitative detection of mouse IL-31 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Mouse
There is no detectable cross-reactivity with other relevant proteins.
mouse IL-31Protein name: Interleukin-31Gene Full Name: interleukin 31abbreviation: IL31Background: Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. The IL31 gene is mapped to chromosome 12q24.31 and the mouse gene maps to a syntenic region on chromosome 5. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. ID:Q6EAL8 ID:76399
IL-31|IL31|IL31_HUMAN|Interleukin-31
Nativ and recombinant mouse IL-31
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
E.coli, T24-C163
<10pg/ml
62.5pg/ml-4000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|387|1228|2155|453|1396|2370 Standard deviation|21.3|78.6|99.13|31.26|100.5|128 CV(%)|5.5|6.4|4.6|6.9|7.2|5.4
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse IL-31 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-31 has been precoated onto 96-well plates. Standards(E.coli, T24-C163) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-31 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-31 amount of sample captured in plate.
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse IL-31 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse IL-31 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.033|0.081|0.130|0.220|0.408|0.793|1.392|2.219
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse IL-31 antibody|1ST0000-10|Lyophilized recombinant mouse IL-31 standard|10ng/tube�2AR1107|Biotinylated anti- mouse IL-31 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Mouse |
Antigen Name | Interleukin-31 (IL31) |
Detection Range | 62.5pg/ml-4000pg/ml |
Alias | IL-31, IL31, IL31_HUMAN, Interleukin-31 |