Mouse IL-27 p28 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL-27 p28 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse IL-27 p28
Protein name: Interleukin-27 subunit alpha
Gene Full Name: interleukin 27
abbreviation: IL27
Background: Interleukin-27(IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus(EBV)-induced gene 3(EBI3)(also known as IL-27B) and IL27-p28(known as IL-30). IL-27 is produced by antigen-presenting cells. By genomic sequence analysis, the IL27 p28 gene was mapped to chromosome 16p11. L-27 plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130.
ID:Q8K3I6
ID:246779

alias

IL 27A| IL 30| IL-27 subunit alpha| IL-27-A| IL27| IL27-A| IL27A| IL27A_HUMAN| IL27p28| IL30| Interleukin 27| Interleukin 27 subunit alpha| Interleukin 30| Interleukin-27 subunit alpha| p28

Origin

Nativ and recombinant mouse IL-27 p28

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, F29-S234

sensitivity

<10pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|103|368|556|127|407|674
Standard deviation|5.87|25.76|36.7|8.13|32.97|51.9
CV(%)|5.7|7|6.6|6.4|8.1|7.7

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL-27 p28 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-27 p28 has been precoated onto 96-well plates. Standards(NSO, F29-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-27 p28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-27 p28 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml mouse IL-27 p28 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse IL-27 p28 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.011|0.037|0.092|0.122|0.270|0.644|1.442|2.317

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL-27 p28 antibody|1
ST0000-1|Lyophilized recombinant mouse IL-27 p28 standard|1ng/tube�2
AR1107|Biotinylated anti- mouse IL-27 p28 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4