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Boster
For quantitative detection of mouse IL-21 in cell culture supernates and serum.
Mouse
There is no detectable cross-reactivity with other relevant proteins.
Mouse IL-21Protein name: Interleukin-21Gene Full Name: interleukin 21abbreviation: IL21Background: Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer(NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells. ID:Q9ES17 ID:60505
Cytokine CX2| DN 452A22.7| IL 21| Interleukin 21| Interleukin 21 isoform| Interleukin21| OTTMUSP00000007838| Za11
Nativ and recombinant mouse IL-21
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
E.coli, P25-S146
<10pg/ml
62.5pg/ml-4000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|316|1296|2113|427|1450|2485 Standard deviation|15.8|80.4|99.31|25.2|97.15|159 CV(%)|5|6.2|4.7|5.9|6.7|6.4
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse IL-21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-21 has been precoated onto 96-well plates. Standards(E.coli, P25-S146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-21 amount of sample captured in plate.
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse IL-21 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse IL-21 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.061|0.093|0.131|0.239|0.479|0.837|1.677|2.361
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse IL-21antibody|1ST0000-10|Lyophilized recombinant mouse IL-21 standard|10ng/tube�2AR1107|Biotinylated anti- mouse IL-21 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Mouse |
Antigen Name | Interleukin-21 (IL21) |
Detection Range | 62.5pg/ml-4000pg/ml |
Alias | Cytokine CX2, DN 452A22.7, IL 21, Interleukin 21, Interleukin 21 isoform, Interleukin21, OTTMUSP00000007838, Za11 |