Mouse IL-20 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL-20 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

mouse IL-20
Protein name: Interleukin-20
Gene Full Name: interleukin 20
abbreviation: IL20
Background: IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.
ID:Q9JKV9
ID:58181

alias

Cytokine Zcyto10|Four alpha helix cytokine|IL-20|IL10D|IL20|IL20_HUMAN|Interleukin 20|Interleukin-20|MGC96907|UNQ852/PRO1801|ZCYTO10

Origin

Nativ and recombinant mouse IL-20

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, L25-E176

sensitivity

<10pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|285|709|1126|343|762|1280
Standard deviation|11.97|36.16|61.93|19.55|48.77|94.72
CV(%)|4.2|5.1|5.5|5.7|6.4|7.4

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL-20 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-20 has been precoated onto 96-well plates. Standards(E.coli, L25-E176) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-20 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-20 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse IL-20 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse IL-20 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.120|0.186|0.231|0.337|0.563|0.892|1.603|2.248

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL-20 antibody|1
ST0000-10|Lyophilized recombinant mouse IL-20 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse IL-20 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4