Mouse IL-2 ELISA Kit

Brand

Cell Sciences

Description

IL-2 is a protein of 133 amino acids (15.4 kDa). It is produced mainly by T-cells expressing the surface antigen CD4 following cellactivation by mitogens or allogens under physiological conditions. IL-2 displays significant anti-tumor activity for a variety of tumorcell types since it supports the proliferation and clonal expansion of T-cells that specifically attack certain tumor types. Human IL-2is active on mouse cells, mouse IL-2 is species-specific and is inactive on human cells. At the amino acid sequence level, there isapproximately 60% similarity between mouse and human IL-2.Cell Sciences Mouse IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbentassay for the quantitative measurement of mouse IL-2 in serum, plasma and cell culture supernatants. This assay employs anantibody specific for mouse IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in asample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-2 antibody isadded. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells areagain washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. TheStop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

spezie

Mouse

Cross Reaction

Mouse

detection target

Mouse IL-2
Antigen: IL2

alias

interleukin-2, aldesleukin, T cell growth factor, involved in regulation of T-cell clonal expansion

sample size

1

pack size

PP

UNSPSC

41116126 and E