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Boster
For quantitative detection of mouse IL-17F in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Mouse
There is no detectable cross-reactivity with other relevant proteins.
Mouse IL-17FProtein name: Interleukin-17FGene Full Name: interleukin 17Fabbreviation: IL17FBackground: Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF. ID:Q7TNI7 ID:257630
CANDF6| Cytokine ML 1| Cytokine ML-1| IL 17F| IL 24| IL-17F| IL-24| Il17f| IL17F_HUMAN| Interleukin 17F| Interleukin 24| Interleukin-17F| Interleukin-24| ML 1| ML1
Nativ and recombinant mouse IL-17F
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
E.coli, R21-A153
<10pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|307|662|1017|345|759|1283 Standard deviation|16.58|41.71|48.82|21.05|56.93|75.7 CV(%)|5.4|6.3|4.8|6.1|7.5|5.9
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse IL-17F ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17F has been precoated onto 96-well plates. Standards(E.coli, R21-A153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17F is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17F amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse IL-17F standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse IL-17F standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.003|0.067|0.140|0.282|0.585|1.12|1.76|2.209
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse IL-17F antibody|1ST0000-10|Lyophilized recombinant mouse IL-17F standard|10ng/tube�2AR1107|Biotinylated anti- mouse IL-17F antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Mouse |
Antigen Name | Interleukin-17F (IL17F) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | CANDF6, Cytokine ML 1, Cytokine ML-1, IL 17F, IL 24, IL-17F, IL-24, Il17f, IL17F_HUMAN, Interleukin 17F, Interleukin 24, Interleukin-17F, Interleukin-24, ML 1, ML1 |