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Boster
For quantitative detection of mouse IL-17D in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Mouse
There is no detectable cross-reactivity with other relevant proteins.
Mouse IL-17DProtein name: Interleukin-17DGene Full Name: interleukin 17Dabbreviation: IL17DBackground: Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas. ID:Q8K4C4 ID:239114
IL 17D|IL 22|IL 27|IL-17D|IL-27|IL17D|IL17D_HUMAN|IL27|Interleukin 17D|Interleukin 27|Interleukin-17D|Interleukin-27
Nativ and recombinant mouse IL-17D
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
E.coli, A25-R205
<10pg/ml
62.5pg/ml-4000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|715|1410|2703|694|1582|2691 Standard deviation|30.03|64.86|137.9|38.17|99.67|180.3 CV(%)|4.2|4.6|5.1|5.5|6.3|6.7
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse IL-17D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17D has been precoated onto 96-well plates. Standards(E.coli, A25-R205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17D amount of sample captured in plate.
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse IL-17D standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse IL-17D standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.042|0.106|0.158|0.253|0.460|0.883|1.530|2.288
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse IL-17D antibody|1ST0000-10|Lyophilized recombinant mouse IL-17D standard|10ng/tubex2AR1107|Biotinylated anti- mouse IL-17D antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Mouse |
Antigen Name | Interleukin-17D (IL17D) |
Detection Range | 62.5pg/ml-4000pg/ml |
Alias | IL 17D, IL 22, IL 27, IL-17D, IL-27, IL17D, IL17D_HUMAN, IL27, Interleukin 17D, Interleukin 27, Interleukin-17D, Interleukin-27 |