Details
Mouse IFN-gamma ELISA Kit
Brand
Cell Sciences
Description
The Cell Sciences Mouse IFN-gamma ELISA (Enzyme-Linked Immunosorbent Assay) kit is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of mouse IFN-gamma in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse IFN-gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IFN-gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Specificity
Mouse IFN-gamma
Sensitivity
< 5 pg/ml
Sample Types
Serum, plasma, and cell culture supernatants
spezie
Mouse
Cross Reaction
Mouse
detection target
Mouse IFN-gamma Antigen: IFN-gamma
alias
IFG, IFI, interferon gamma, IFN-gamma, immune interferon
sensitivity
< 5 pg/ml
sample size
1
pack size
PP
UNSPSC
41116126 and E