Mouse Galectin-3 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse Galectin-3 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Galectin-3 in mouse serum, plasma and cell culture media. An antibody specific for mouse Galectin-3 was coated on a 96-well plate. Standards and samples are added to the wells and any Galectin-3 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Galectin-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Galectin-3 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker;

specific against

Mouse

detection target

lectin, galactoside-binding, soluble, 3( Galectin-3 )
Background: Galectin-3 is a protein that in humans is encoded by the LGALS3 gene. Galectin-3 is a member of the lectin family, of which 14 mammalian galectins have been identified. The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. Galectin-3 (Gal-3) is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis.

alias

CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2

sensitivity

4 pg/ml

detection range

8.2-2000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P16110