Mouse Galectin-1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse Galectin-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Galectin-1 in mouse serum, plasma and cell culture media. An antibody specific for mouse Galectin-1 was coated on a 96-well plate. Standards and samples are added to the wells and any Galectin-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Galectin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Galectin-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Hematologic Cancer;

specific against

Mouse

detection target

lectin, galactose binding, soluble 1(Galectin-1)
Background: The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells.

alias

AA410090, Gal-1, Galbp, L-14.5, L14, Lect14, galectin-1

sensitivity

10 pg/ml

detection range

16.4-4000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P16045