BioAim Scientific (Kanada)
The Bioaim Mouse Epiregulin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Epiregulin in mouse serum, plasma and cell culture media. An antibody specific for mouse Epiregulin was coated on a 96-well plate. Standards and samples are added to the wells and any Epiregulin present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Epiregulin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Cell growth; proliferation;
epiregulin (Epiregulin) Background: Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), Transforming Growth Factor (TGF)-alpha, Amphiregulin (AR), HB (heparin-binding)-EGF, Betacellulin (BTC), and the various heregulins (HRGs). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB family receptors (ErbB1 through B4). Epiregulin is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
Serum, plasma, Cell culture supernatant
|Detection Range||8.2-6000 pg/ml|