Mouse CD30 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse CD30 in cell culture supernates and serum.

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse CD30
Protein name: Tumor necrosis factor receptor superfamily member 8
Gene Full Name: tumor necrosis factor receptor superfamily, member 8
abbreviation: TNFRSF8
Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
ID:Q60846
ID:21941

alias

CD 30|CD153|CD30|CD30 antigen|CD30L receptor|Cytokine receptor CD30|D1S166E|KI 1|Ki 1 antigen|Ki-1 antigen|KI1|Lymphocyte activation antigen CD30|TNFRSF 8|TNFRSF8|TNFRSF8|TNR8_HUMAN|Tumor Necrosis Factor Receptor Superfamily Member 8|Tumor Necrosis Factor Receptor Superfamily Member 8

Origin

Nativ and recombinant mouse CD30

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, F19-T281

sensitivity

<10pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|90|345|653|102|367|721
Standard deviation|4.77|21.39|46.36|6.63|27.53|60.56
CV(%)|5.3|6.2|7.1|6.5|7.5|8.4

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse CD30 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD30 has been precoated onto 96-well plates. Standards(NSO, F19-T281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD30 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml mouse CD30 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse CD30 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.014|0.081|0.148|0.235|0.505|1.010|1.482|2.158

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse CD30 antibody|1
ST0000-10|Lyophilized recombinant mouse CD30 standard|10ng/tubex2
AR1107|Biotinylated anti- mouse CD30 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4