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Boster
For quantitative detection of mouse BCAM in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Mouse
There is no detectable cross-reactivity with other relevant proteins.
mouse BCAMProtein name: Basal cell adhesion moleculeGene Full Name: basal cell adhesion molecule (Lutheran blood group)abbreviation: BCAMBackground: Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene. ID:Q9R069 ID:57278
AU | Bcam | CD239 | CD239 antigen | F8/G253 antigen | LU | Lutheran | MSK19 | Q9R069
Nativ and recombinant mouse BCAM
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
NSO, E26-A541
<15pg/ml
156pg/ml-10000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|472|758|1380|563|926|1570 Standard deviation|25.49|47.75|93.84|38.84|68.52|128.74 CV(%)|5.4|6.3|6.8|6.9|7.4|8.2
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse BCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BCAM has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E26-A541) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BCAM amount of sample captured in plate.
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse BCAM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse BCAM standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.020|0.084|0.155|0.277|0.494|0.888|1.520|2.123
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse BCAM antibody|1ST0000-10|Lyophilized recombinant mouse BCAM standard|10ng/tube�2AR1107|Biotinylated anti- mouse BCAM antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <15pg/ml |
Specific Against | Mouse |
Antigen Name | mouse BCAM |
Detection Range | 156pg/ml-10000pg/ml |
Alias | AU , Bcam , CD239 , CD239 antigen , F8/G253 antigen , LU , Lutheran , MSK19 , Q9R069 |