Mouse Angiopoietin-2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse Angiopoietin-2 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse Angiopoietin-2
Protein name: Angiopoietin 2
Gene Full Name: angiopoietin 2
abbreviation: ANGPT2
Background: Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
ID:O35608
ID:11601

alias

ANG 2 | ANG2 | ANG-2 | Angiopoietin 2 | Angiopoietin2 | Angiopoietin-2 | Angiopoietin2(ANG2) | Angiopoietin-2(ANG-2) | ANGP2 | ANGPT2 | O15123

Origin

Nativ and recombinant mouse Angiopoietin-2

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

CHO, M1-F496

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.15|3.53|7.21|1.18|3.62|7.34
Standard deviation|0.044|0.148|0.368|0.103|0.308|0.558
CV(%)|3.8|4.2|5.1|8.7|8.5|7.6

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse Angiopoietin-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards(CHO, M1-F496) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Angiopoietin-2 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse Angiopoietin-2 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.002|0.092|0.147|0.312|0.589|1.114|1.672|2.346

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse Angiopoietin-2 antibody|1
ST0000-10|Lyophilized recombinant mouse Angiopoietin-2 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse Angiopoietin-2 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4