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Boster
For quantitative detection of mouse ADAM12 in cell culture supernates, serum and plasma(heparin).
Mouse
There is no detectable cross-reactivity with other relevant proteins.
mouse ADAM12Protein name: Disintegrin and metalloproteinase domain-containing protein 12Gene Full Name: ADAM metallopeptidase domain 12abbreviation: ADAM12Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3. ID:Q61824 ID:11489
ADA12_HUMAN| ADAM 12| ADAM metallopeptidase domain 12| ADAM12| Disintegrin and metalloproteinase domain-containing protein 12| MCMP| MCMPMltna| Meltrin-alpha| MLTN| MLTNA
Nativ and recombinant mouse ADAM12
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
CHO, R29-S513
<10pg/ml
156pg/ml-10000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(ng/ml)|1.45|3.76|6.87|1.54|3.81|7.02 Standard deviation|0.062|0.139|0.24|0.089|0.171|0.365 CV(%)|4.3|3.7|3.5|5.8|4.5|5.2
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse ADAM12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ADAM12 has been precoated onto 96-well plates. Standards(CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ADAM12 amount of sample captured in plate.
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse ADAM12 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse ADAM12 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.023|0.091|0.152|0.291|0.561|0.964|1.723|2.339
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse ADAM12 antibody|1ST0000-10|Lyophilized recombinant mouse ADAM12 standard|10ng/tube�2AR1107|Biotinylated anti- mouse ADAM12 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Mouse |
Antigen Name | Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) |
Detection Range | 156pg/ml-10000pg/ml |
Alias | ADA12_HUMAN, ADAM 12, ADAM metallopeptidase domain 12, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, Meltrin-alpha, MLTN, MLTNA |