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DlDevelop
for quantitatively determining the concentration in the customer's samples Intended Use: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rhesus monkey Simian
Simian
This assay has high sensitivity and excellent specificity for detection of this index.No significant cross-reactivity or interference between this index and analogues was observed.
FSH
1.22 ng/mL
3.125-200 ng/mL
CE and ISO (Nr.:17916Q10078ROS)
48 and 96 Wells
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
1.For unopened kit: All the reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer (30 x concentrate) and the Stop Solution should be stored at 4°C. upon receipt while the others should be at -20°C.2.For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to this index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled this index and unlabeled this index (Standards or samples) with the pre-coated antibody specific to this index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of this index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of this index in the sample.
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 2Standard 2 Standard Diluent 1x20mLDetection Reagent A 1x70µL Assay Diluent A 1x12mLDetection Reagent B 1x120µL Assay Diluent B 1x12mLTMB Substrate 1x9mL Stop Solution 1x6mLWash Buffer (30 x concentrate) 1x20mL Instruction manual 1
Hersteller | DlDevelop |
---|---|
Herstellerland | China |
Sensitivity | 1.22ng/mL |
Specific Against | Monkey |
Detection Range | 3.125-200ng/mL |
Zertifikat | CE and ISO (Nr.:17916Q10078ROS) |