Low Molecular Weight Heparin (LMWH) ELISA for Buffer

Brand

Lifespan Technologies

short description

Heparin is a glycosaminoglycan with alternating uronic acid and aminoglycoside units. It is an anticoagulant used either in its native unfractionated form (UFH) MW ~16 kD or in various partially depolymerized forms (LMWH) of 4-8 kD. The heparin-ELISA product number K-1800 is a quantitative enzyme-linked assay designed for the in vitro measurement of low molecular weight heparin levels in low protein content fluids such as buffer or urine. This assay measures heparin directly using a heparin binding protein which has been conjugated to HRP. The heparin-ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of heparin present in the sample. Samples to be assayed are first mixed with the Detector-Enzyme Conjugate in wells of the heparin coated plate. Heparin in the sample competes with heparin bound to the plate for binding of the Detector-Enzyme Conjugate. The concentration of heparin in the sample is determined using a standard curve of known amounts of heparin.
Pipettes Absorbance microplate reader Plate Cover
Research focus: Cytokines / Growth Factors;

spezie

Human

detection target

Low Molecular Weight Heparin (LMWH)

pack size

1x96 Wells

storage and Stability

Kit can be stored unopened at 4°C for up to six months. Opened solutions can be used for up to one week when stored at 4°C. All components and solutions should be protected from light. Reconstituted detector enzyme conjugate should be used immediately or aliquotted and stored at -80°C.

Kit Content

Heparin-coated 96-well plate Pipettes LMWH (Sigma) Standard (10 µg/ml) Absorbance microplate reader Detector -Enzyme Conjugate vial Plate Cover Conjugate Diluent TMB Solution Stop Solution, 0.5M H2SO4 Wash Concentrate 10X, (dilute 1 part plus 9 parts water to make TBS plus 0.05% TWEEN 20) Standard Diluent, TBS (150 mM NaCl, 10 mM Tris pH 7.5)