Influenza Virus A IgA -

Brand

GENWAY

Overview

Intended Use: The Influenza Virus A IgA-ELISA is intended for the qualitative determination of IgA class antibodies to Influenza virus A in human serum or plasma (citrate). Introduction: Influenza are RNA viruses of the family Orthomyxoviridae. Influenza viruses are divided into three types designated A B and C which are differentiated by the specificity of a soluble antigen associa-ted with the internal ribonucleoprotein component of the virion. The virions are sperical particles of 80-120 nm in diameter consisting of the ribonucleoprotein component and enveloped by matrixprotein and a lipid bilayer which contains two spikeline structures: viral hemagglutinin (H) and viral neuraminidase (N). Influenza viruses are respiratory tract pathogens which are transmitted by direct contact large-droplet infection or by contaminated surfaces. Influenzy types A and B are responsible for epidemics of respiratory illness that occur almost every winter and are often associated with increased rates of hospitalization and death. Type C infections usually cause either a very mild respiratoryillness or no symptoms at all; it does not cause epidemics and does not have the severe public health impact that influenza types A and B do. Pandemics of influenza virus type A infections have occured at 10 to 20 year intervals since 1890. Apparently this results from alterations in the composition of the H and N antigens (antigenic ?drift? and antigenic ?shift?). Currently two subtypes of influenza A are circulating worldwide. Normally influenza is a self-limiting disease lasting for 3 to 7 days but some people develop serious and potentially life-threatening medical complications such as pneumonia particularly in children elderly people and other vulnerable groups. The presence of virus resp. infection may be identified byMicroscopy: CPE IFPCRSerology: Hemadsorption Hemagglutination-Inhibition Detection of antibody production by ELISAPrinciples of the assay: The qualitative immunoenzymatic determination of IgA-class antibodies to Influenza Virus A is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Influenza Virus A antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgA conjugate is added. This conjugate binds to the captured Influenza Virus A specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Influenza Virus A specific IgA antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader. Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2. . . 8 °C. Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history symptomatology as well as serological data. In immunocompromised patients and newborns serological data only have restricted value. References Klenk mH. D. R. Rott: The molecular biology of influenza virus pathogenicity. Adv. Virus. Res. 34 (1988) 247- 281Palese P. D. K. Kingsburg (Hrsg. ):Genetics of influenza viruses. Springer Wien New York (1983). Webster R. G. W. J. Bean O. T. Gorman et al. : evolution and ecology of influenza A viruses. Microbiol. Rev. 56 (1992) 152-179Assaad F. G. Torrigiani:immunological aspects of thze prevention of viral diseases. Bull. World Health Organ 65 (1987) 1-10Couch R. B. J. A. Kasal W. P. Glezen et al. : Influenza:its control in persons and population. J. Infect. Dis. 153 (1986) 431-440

spezie

other

detection target

Influenza Virus A IgA

pack size

1x96 assay