The Influenza A H7N7 HA ELISA Pair Set is for the quantitative determination of H7N7 HA. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for InfluenzaA H7N7 HA coated on a 96-well plate. Standards and samples are added to thewells, and any H7N7 HA present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated rabbit anti-H7N7 HA polyclonalantibody is then added, producing an antibody-antigen-antibody "sandwich". Thewells are again washed and TMB substrate solution is loaded, which producescolor in proportion to the amount of H7N7 HA present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwellare read at 450 nm.
HAStandard (antigene): Each vial contains 40 ng of recombinant influenza A H7N7 HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended capture antibody type: 1.0 mg/mL of rabbit anti-H7N7 HA monoclonal antibody. Dilute to a working concentration of 0.5 µg/mL in CBS before coating. (Catalog: # 11082-R002)detection antibody type: 0.5 mg/mL rabbit anti-H7N7 HA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 2 µg/mL in detection antibody dilution buffer before use.Lable: HRP
The minimum detectable dose of Influenza A H7N7 HA was determined to be approximately 46.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Influenza A H7N7 HA was determined to be approximately 46.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Antigen Name||Influenza A H7N7 Hemagglutinin|
|Detection Range||46.8 pg/ml|
|Alias||Influenza A H7N7 Hemagglutinin / HA|