The H3N2 (A/Brisbane/10/2007) HA ELISA Pair Set is for the quantitative determination of H3N2 HA . This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H3N2 ( A/Brisbane/10/2007) HA coated on a 96-well plate. Standards and samples areadded to the wells, and any H3N2 (A/Brisbane/10/2007) HA present binds to theimmobilized antibody. The wells are washed and a horseradish peroxidaseconjugated mouse anti-H3N2 HA monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof H3N2 (A/Brisbane/10/2007) HA present in the sample. To end the enzymereaction, the stop solution is added and absorbances of the microwell are readat 450 nm.
The following factors were assayed and exhibited cross-reactivity with H3N2 (A/Wisconsin/67/X-161/2005) HA H3N2 (A/Wyoming/3/2003) HA H3N2 (A/Aichi/2/68) HA
HAStandard (antigene): Each vial contains 6500 ng of recombinant H3N2 (A/Brisbane/10/2007) HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 40000 pg/mL is recommended capture antibody type: 0.25 mg/mL of rabbit anti-(A/Brisbane/10/2007) monoclonal antibody. Dilute to a working concentration of 2.0 µg/mL in CBS before coating (Catalog: # 11056-R014)detection antibody type: 0.5 mg/mL mouse anti-H3N2 (A/Brisbane/10/2007) HA monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 µg/mL in detection antibody dilution buffer before use. (Catalog: # 11056-MM01)Lable: HRP
The minimum detectable dose of H3N2 (A/Brisbane/10/2007) HA was determined to be approximately 625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of H3N2 (A/Brisbane/10/2007) HA was determined to be approximately 625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Antigen Name||Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin|
|Detection Range||625 pg/ml|
|Alias||Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin / HA|