The human Transferrin / TF ELISA Pair Set is for the quantitative determination of human Transferrin . This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific forTransferrin / TF coated on a 96-well plate. Standards and samples are added tothe wells, and any Transferrin / TF present binds to the immobilized antibody.The wells are washed and a horseradish peroxidase conjugated mouse anti-Transferrin / TF monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof Transferrin / TF present in the sample. To end the enzyme reaction, the stopsolution is added and absorbances of the microwell are read at 450 nm.
TFStandard (antigene): Each vial contains 70 ng of recombinant Transferrin. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended capture antibody type: 0.5 mg/mL of mouse anti-Transferrin / TF monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating (Catalog: # 11019-MM01)detection antibody type: 0.4 mg/mL mouse anti-Transferrin / TF monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 µg/mL in detection antibody dilution buffer before use. (Catalog: # 11019-MM06)Lable: HRP
The minimum detectable dose of human Transferrin / TF was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of human Transferrin / TF was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard|
|Detection Range||15.6 pg/ml|
|Alias||Transferrin / TF|