Human TNFRSF9/4-1BB PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human TNFRSF9 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human TNFRSF9
Protein name: Tumor necrosis factor receptor superfamily member 9
Gene Full Name: tumor necrosis factor receptor superfamily, member 9
abbreviation: TNFRSF9
Background: CD137 is a member of the tumor necrosis factor(TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9(TNFRSF9), 4-1BB and induced by lymphocyte activation(ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.
ID:Q07011
ID:3604

alias

4-1BB|4-1BB ligand receptor|CD137|CD137 antigen|CDw137|Homolog of mouse 4 1BB|ILA|Induced by lymphocyte activation (ILA)|Interleukin activated receptor homolog of mouse Ly63|Receptor protein 4 1BB|T-cell antigen 4-1BB homolog|T-cell antigen ILA|TNFRSF9|TNR9_HUMAN|Tumor necrosis factor receptor superfamily member 9

Origin

Nativ and recombinant human TNFRSF9

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, L24-Q186

sensitivity

<10pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|241|668|1134|224|735|1268
Standard deviation|8.9|34|48.8|11|45.57|69.8
CV(%)|3.7|5.1|4.3|4.9|6.2|5.5

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human TNFRSF9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF9 has been precoated onto 96-well plates. Standards(NSO, L24-Q186) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF9 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human TNFRSF9 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human TNFRSF9 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.010|0.091|0.171|0.350|0.627|1.050|1.660|2.217

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human TNFRSF9 antibody|1
ST0000-10|Lyophilized recombinant human TNFRSF9 standard|10ng/tube�2
AR1107|Biotinylated anti- human TNFRSF9 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4