Human TNFRSF16/NGFR PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human TNFRSF16 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human TNFRSF16
Protein name: Tumor necrosis factor receptor superfamily member 16
Gene Full Name: nerve growth factor receptor
abbreviation: NGFR
Background: Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.
ID:P08138
ID:4804

alias

CD271|CD271 antigen|Gp80 LNGFR|Gp80 LNGFR|Gp80-LNGFR|Low affinity nerve growth factor receptor|Low affinity nerve growth factor receptor|Low affinity neurotrophin receptor p75NTR|Low affinity neurotrophin receptor p75NTR|Low-affinity nerve growth factor receptor|Nerve growth factor receptor|Nerve growth factor receptor TNFR superfamily member 16|NGF receptor|NGF receptor|NGFR|p75 ICD| p75 ICD|p75 Neurotrophin receptor|p75 NTR|p75(NTR)|p75NTR|TNFR Superfamily Member 16|TNFRSF16|TNR16_HUMAN|Tumor necrosis factor receptor superfamily member 16

Origin

Nativ and recombinant human TNFRSF16

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

sf21, K29-N250

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|81|330|1560|85|360|1350
Standard deviation|4.21|21.45|90.48|5.27|27|90.45
CV(%)|5.2|6.5|5.8|6.2|7.5|6.7

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human TNFRSF16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF16 has been precoated onto 96-well plates. Standards (sf21, K29 - N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF16 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human TNFRSF16 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human TNFRSF16 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.009|0.057|0.129|0.205|0.416|0.685|1.375|2.203

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human TNFRSF16 antibody|1
ST0000-10|Lyophilized recombinant human TNFRSF16 standard|10ng/tube�2
AR1107|Biotinylated anti- human TNFRSF16 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4