Human TNF-beta ELISA Kit

Brand

Cell Sciences

Description

Human TNF-beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbentassay for the quantitative measurement of human TNF-beta in serum, plasma, cell culture supernatants and urine.This assay employs an antibody specific for human TNF-beta coated on a 96-well plate. Standards and samples arepipetted into the wells and TNF-beta present in a sample is bound to the wells by the immobilized antibody. The wellsare washed and biotinylated anti-human TNF-beta antibody is added. After washing away unbound biotinylatedantibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solutionis added to the wells and color develops in proportion to the amount of TNF-beta bound. The Stop Solution changesthe color from blue to yellow, and the intensity of the color is measured at 450 nm.

host

Rabbit

spezie

Human

Cross Reaction

rhesus monkey, cynomolgous monkey

detection target

Human TNF-beta
Antigen: TNF-beta
Antibody Type: Polyclonal

alias

LT, TNFB, TNFSF1, OTTHUMP00000029184, OTTHUMP00000037612, OTTHUMP00000037613, OTTHUMP00000165897, lymphotoxin alpha, tumor necrosis factor beta

sample size

1

pack size

PP

UNSPSC

41116126 and E