Human TNF-alpha ELISA Kit

Brand

Cell Sciences

Description

Tumor Necrosis Factor alpha (TNF alpha) is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial lipopolysaccharides (LPS). TNF alpha shows a wide spectrum of biological activities. It causes cytolysis and cytostasis of many tumor cell lines in vitro . Within hours after injection TNF alpha leads to the destruction of small blood vessels within malignant tumors. TNF alpha also enhances phagocytosis and cytotoxicity in neutrophilic granulocytes and also modulates the expression of many other proteins. The Human TNF alpha ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human, TNF alpha in serum, plasma, cell culture superntants and urine. This assay employs an antibody specific for Human TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human TNF alpha antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound.

Storage and Stability

May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.

Sensitivity

The minimum detectable dose of TNF alpha is typically less than 15 pg/mL.

spezie

Human

Cross Reaction

Human

detection target

Human TNF-alpha
Antigen: TNF-alpha

alias

DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, tumor necrosis factor, TNF-a, cachectin, APC1 protein, TNF, monocyte-derived, TNF, macrophage-derived, TNF superfamily, member 2, tumor necrosis factor alpha, tumor necrosis factor ligand superfamily member 2

sensitivity

The minimum detectable dose of TNF alpha is typically less than 15 pg/mL.

sample size

1

pack size

PP

UNSPSC

41116126 and E