Human TNF-alpha ELISA

Brand

Raybiotech

short description

RayBio® Human TNF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.

specific against

Human

Cross Reaction

Human

detection target

TNF alpha

alias

Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF); Intracellular domain 1 (ICD1); Intracellular domain 2 (ICD2); C-domain 1; C-domain 2; Tumor necrosis factor, soluble form]

sensitivity

30 pg/ml

detection range

30 pg/ml - 6000 pg/ml

certificate

GMP/ISO

pack size

1x96, 2x96 and 5x96 Wells Microplate Kit

Reproducibility

Intra-Assay CV%: <10%; Inter-Assay CV%: <12%

storage and Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Principle of the Elisa

Sandwich ELISA kits - Sandwich-based

Kit Content

Pre-Coated 96-well Strip Microplate; Wash Buffer; Stop Solution; Assay Diluent(s); Lyophilized Standard; Biotinylated Detection Antibody; Streptavidin-Conjugated HRP; TMB One-Step Substrate