Human SOST ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human SOST ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of SOST in Human serum, plasma and cell culture media. An antibody specific for Human SOST was coated on a 96-well plate. Standards and samples are added to the wells and any SOST present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human SOST antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of SOST present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Embryogenesis and morphogenesis;

specific against

Human

detection target

Sclerostin(SOST)
Background: Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. Although the underlying mechanisms are unclear, it is believed that the antagonism of BMP-induced bone formation by sclerostin is mediated by Wnt signaling, but not BMP signaling pathways. Sclerostin is expressed in osteocytes and some chondrocytes and it inhibits bone formation by osteoblasts. Sclerostin production by osteocytes is inhibited by parathyroid hormone, mechanical loading and cytokines including prostaglandin E2, oncostatin M, cardiotrophin-1 and leukemia inhibitory factor. Sclerostin production is increased by calcitonin. Thus, osteoblast activity is self regulated by a negative feedback system.

alias

CDD, DAND61, VBCH, SOST

sensitivity

30 pg/ml

detection range

40.9-10000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q9BQB4