Human soluble CD5 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD5 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD5
Protein name: T-cell surface glycoprotein CD5
Gene Full Name: CD5 molecule
abbreviation: CD5
Background: CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli(such as bacterial proteins) and not by normal tissue proteins.
ID:P06127
ID:921

alias

CD 5|CD5|CD5 antigen (p56 62)|CD5 antigen|CD5 molecule|CD5_HUMAN|LEU 1|LEU1|Ly12|LyA|Lymphocyte Antigen CD5|Lymphocyte antigen T1/Leu 1|Lymphocyte antigen T1/Leu-1|Lymphocyte glycoprotein T1/Leu1|OTTHUMP00000236973|p56 62|T cell surface glycoprotein CD5|T-cell surface glycoprotein CD5|T1

Origin

Nativ and recombinant human CD5

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, R25-N371

sensitivity

<10pg/ml

detection range

93.7pg/ml-6000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.37|0.85|4.27|0.38|0.92|4.31
Standard deviation|0.023|0.041|0.167|0.031|0.699|0.311
CV(%)|6.2|4.8|3.9|8.2|7.6|7.2

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD5 has been precoated onto 96-well plates. Standards(NSO, R25-N371) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD5 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.7pg/m human CD5 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human CD5 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|93.7|187.5|375|750|1500|3000|6000 O.D.|0.023|0.114|0.235|0.420|0.776|1.201|1.710|2.221

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD5 antibody|1
ST0000-10|Lyophilized recombinant human CD5 standard|10ng/tube�2
AR1107|Biotinylated anti- human CD5 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4