BioAim Scientific (Kanada)
The Bioaim Human sgp130 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of sgp130 in Human serum, plasma and cell culture media. An antibody specific for Human sgp130 was coated on a 96-well plate. Standards and samples are added to the wells and any sgp130 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human sgp130 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of sgp130 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
interleukin 6 signal transducer(Sgp130) Background: Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the classI cytokine receptor family. Binding of IL-6 or IL-11 to either the membrane-anchored or soluble IL-6 R or IL-11 R, respectively, initiates the association of IL-6 R (IL-11 R) with gp130. The complex then undergoes homodimerization and signal transduction. For other IL-6 family cytokines, such as LIF and OSM, signal transduction is similarly triggered by the heterodimerization of gp130 with LIFR or OSM R. gp130 has no intrinsic tyrosine kinase activity. Instead, it is phosphorylated on tyrosine residues after complexing with other proteins. The phosphorylation leads to association with JAK/Tyk tyrosine kinases and STAT protein transcription factors. In particular, STAT-3 is activated which leads to the activation of many downstream genes. Other pathways activated include RAS and MAPK signaling.
CD130, CDW130, GP130, IL-6RB
Serum, plasma, Cell culture supernatant
|Antigen Name||interleukin 6 signal transducer|
|Detection Range||8.2-6000 pg/ml|
|Alias||CD130, CDW130, GP130, IL-6RB|