Human NT-3 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human NT-3 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of NT-3 in human serum and plasma. An antibody specific for human NT-3 was coated on a 96-well plate. Standards and samples are added to the wells and any NT-3 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human NT-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of NT-3 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Neuronal differentiation and survival;

specific against

Human

detection target

neurotrophin 3( NT-3 )
Background: Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides; which are proteolytically processed to generate the mature proteins. Similarly to NGF, bioactive NT-3 is predicted to be a noncovalently linked homodimer. NT-3 is unique in the number of neurons it can potentially stimulate, given its ability to activate two of the receptor tyrosine kinase neurotrophin receptors .

alias

HDNF, NGF-2, NGF2, NT-3, NT3

sensitivity

2 pg/ml

detection range

4.1-3000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P20783