Human IL7R/CD127 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL7R in cell culture supernates and serum.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL7R
Protein name: Interleukin-7 receptor subunit alpha
Gene Full Name: interleukin 7 receptor
abbreviation: IL7R
Background: The interleukin-7 receptor(IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7(IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain(IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V(D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
ID:P16871
ID:3575

alias

CD 127|CD127|CD127 antigen|CDw127|IL 7R alpha|IL 7R|IL-7 receptor subunit alpha|IL-7R subunit alpha|IL-7R-alpha|IL-7RA|IL7R|IL7RA|IL7RA_HUMAN|IL7Ralpha|ILRA|Interleukin 7 receptor alpha chain|Interleukin 7 receptor|Interleukin 7 receptor isoform H5 6|Interleukin-7 receptor subunit alpha

Origin

Nativ and recombinant human IL7R

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, E21-I262

sensitivity

<10pg/ml

detection range

312pg/ml-20000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|2.8|8|14.3|3.4|9.1|16.2
Standard deviation|0.168|0.36|0.815|0.245|0.51|1.07
CV(%)|6|4.5|5.7|7.2|5.6|6.6

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL7R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL7R has been precoated onto 96-well plates. Standards(NSO, E21-I262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL7R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL7R amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human IL7R standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL7R standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000 O.D.|0.037|0.158|0.285|0.502|0.855|1.315|1.796|2.299

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL7R antibody|1
ST0000-20|Lyophilized recombinant human IL7R standard|20ng/tube�2
AR1107|Biotinylated anti- human IL7R antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4