Human IL1RL1/ST2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL1RL1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human IL1RL1
Protein name: Interleukin-1 receptor-like 1
Gene Full Name: interleukin 1 receptor-like 1
abbreviation: IL1RL1
Background: Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I(IL1R1), interleukin 1 receptor, type II(IL1R2) and interleukin 1 receptor-like 2(IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
ID:Q01638
ID:9173

alias

DER-4|DER4|FIT 1|Growth stimulation expressed|homolog of mouse growth stimulation-expressed|Il1rl1|IL33R|ILRL1_HUMAN|Interleukin 1 receptor like 1|interleukin 1 receptor related protein|Interleukin-1 receptor-like 1|Ly84|Lymphocyte antigen 84|Protein ST2|ST2|ST2L|ST2V|Ste2|Suppressor of tumorigenicity 2|T1|T1 protein

Origin

Nativ and recombinant human IL1RL1 as well as free IL1RL1 and IL-33 complexed IL1RL1.

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, K19-S328

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.53|2.05|4.31|0.69|2.54|4.68
Standard deviation|0.028|0.094|0.142|0.06|0.157|0.229
CV(%)|5.2|4.6|3.3|8.7|6.2|4.9

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL1RL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1RL1 has been precoated onto 96-well plates. Standards(NSO, K19-S328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1RL1 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human IL1RL1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL1RL1 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.006|0.083|0.158|0.327|0.568|1.074|1.823|2.311

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL1RL1 antibody|1
ST0000-10|Lyophilized recombinant human IL1RL1 standard|10ng/tube�2
AR1107|Biotinylated anti- human IL1RL1 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4