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Boster
For quantitative detection of human IL1R2 in cell culture supernates and serum.
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL1R2Protein name: Interleukin-1 receptor type 2Gene Full Name: interleukin 1 receptor, type IIabbreviation: IL1R2Background: Interleukin 1 receptor, type II(IL1R2) also known as CD121b(Cluster of Differentiation 121b) is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. It is mapped to 2q11.2. IL1R2 binds interleukin alpha(IL1A), interleukin beta(IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. The secreted IL1R2 recruits secreted IL1RAP with high affinity, and this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. ID:P27930 ID:7850
IL1 Receptor II|Antigen CDw121b|CD121|CD121 antigen-like family member B|CD121b|CD121B Antigen|CDw121b|IL-1R-2|IL-1R-beta|IL-1R2|IL-1RT-2|IL-1RT2|IL1 R2|IL1 receptor beta chain|IL1R beta|Il1r-2|IL1R2|IL1R2_HUMAN|IL1RB|Interleukin 1 receptor type II precursor|Interleukin 1 receptor, beta, type II|Interleukin 1 receptor, type II|Interleukin-1 receptor beta|Interleukin-1 receptor type 2|Interleukin-1 receptor type II|MGC137383|MGC47725|OTTHUMP00000207969|p68|Type II interleukin 1 receptor beta|Type II interleukin-1 receptor, beta
Nativ and recombinant human IL1R2
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
sf21, F14-E343
<10pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|85|392|750|96|414|763 Standard deviation|3.91|12.2|28.5|5.95|22.77|38.9 CV(%)|4.6|3.1|3.8|6.2|5.5|5.1
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL1R2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1R2 has been precoated onto 96-well plates. Standards(sf21, F14-E343) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R2 amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human IL1R2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL1R2 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.066|0.088|0.144|0.254|0.544|1.007|1.821|2.388
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL1R2 antibody|1ST0000-10|Lyophilized recombinant human IL1R2 standard|10ng/tubex2AR1107|Biotinylated anti- human IL1R2 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
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Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-1 receptor type 2 (IL1R2) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | IL1 Receptor II, Antigen CDw121b, CD121, CD121 antigen-like family member B, CD121b, CD121B Antigen, CDw121b, IL-1R-2, IL-1R-beta, IL-1R2, IL-1RT-2, IL-1RT2, IL1 R2, IL1 receptor beta chain, IL1R beta, Il1r-2, IL1R2, IL1R2_HUMAN, IL1RB, Interleukin 1 rece |